LOCUS       pLV-[hsa-miR-375]        9118 bp    DNA     circular      1-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7039..7899
                     /label=Amp(R)
     promoter        8100..8640
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5644..5878
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6453..6908)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5950..6080
                     /label=SV40 pA
     misc_feature    6081..6294
                     /label=SV40 ori
     misc_feature    4965..5561
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3583
                     /label=intron
     CDS             3630..4955
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3373
                     /label=hsa-mir-375 precursor
BASE COUNT     2139 a      2407 c      2498 g      2074 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gcggagccta 
     3121 gcggggcgct gtgcagcact gagctcgcgg aagaccagga ccaggagatc accgagggcg 
     3181 accgccaggc cccgggccct ccgctcccgc cccgcgacga gcccctcgca caaaccggac 
     3241 ctgagcgttt tgttcgttcg gctcgcgtga ggcaggggcg gcctctcagc accagcccgg 
     3301 ggggcggcct gatcgccacg caggcacctg ccgccgccac cgccaccgcc atctcaaccg 
     3361 tacgggtggg agagctagct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg 
     3421 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg 
     3481 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct 
     3541 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaaaactacc 
     3601 cctctagagt cgagctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg 
     3661 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg 
     3721 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 
     3781 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca 
     3841 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt 
     3901 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga 
     3961 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg 
     4021 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct 
     4081 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga 
     4141 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc 
     4201 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg 
     4261 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca 
     4321 tggacgagct gtacaaggac ccaccggtcg ccaccatgac cgagtacaag cccacggtgc 
     4381 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 
     4441 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 
     4501 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 
     4561 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 
     4621 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 
     4681 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 
     4741 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 
     4801 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 
     4861 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg 
     4921 cgacctggtg catgacccgc aagcccggtg cctgagcggc cgcgtcgaca atcaacctct 
     4981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 
     5041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 
     5101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 
     5161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 
     5221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 
     5281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 
     5341 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 
     5401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 
     5461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 
     5521 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct 
     5581 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 
     5641 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg 
     5701 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 
     5761 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 
     5821 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 
     5881 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 
     5941 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     6001 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     6061 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc 
     6121 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     6181 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     6241 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 
     6301 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 
     6361 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 
     6421 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 
     6481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     6541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     6601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
     6661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     6721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     6781 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     6841 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 
     6901 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 
     6961 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 
     7021 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 
     7081 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 
     7141 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 
     7201 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 
     7261 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 
     7321 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 
     7381 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 
     7441 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 
     7501 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 
     7561 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 
     7621 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 
     7681 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 
     7741 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 
     7801 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 
     7861 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 
     7921 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 
     7981 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     8041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     8101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     8161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 
     8221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     8281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     8341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     8401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     8461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     8521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     8581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     8641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     8701 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 
     8761 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 
     8821 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 
     8881 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 
     8941 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 
     9001 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 
     9061 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgc 
//
